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Radiance® Label-Free Monitoring of Adenovirus Infection of HEK293 Cells

In this technical note, cells were infected two hours post seeding and then harvested for analysis with Radiance at 48 hours post infection in two separate experiments in order to demonstrate LFC’s ability to quantify adenovirus infection in HEK 293 cells. Cells were infected at multiplicities of infection (MOIs) of 0.00, 0.082, 0.25, 0.74, 2.22, 6.67, and 20.00 (IU/cell8) to generate a correlation curve between Radiance data and viral titers. As each cell flows through the microfluidic channel, the laser within Radiance exerts an optical force on the cell, reducing its velocity…

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