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Radiance® Label-Free Monitoring of AAV Transfection in HEK293 Cells Using Laser Force Cytology™ (LFC™)

In this technical note, collaborative efforts are described between Catalent and LumaCyte to compare AAV production with three different transfection reagents using both LFC and a digital droplet PCR (ddPCR) based viral genome titer assay. A strong correlation was generated between the LFC based measurements, which are available in near real-time (5 minutes analysis time per sample) and the ddPCR results which take significant time and labor, demonstrating the utility of LFC for rapid process monitoring. Additional applications of LFC throughout the AAV production process include adventitious agent monitoring to rapidly detect potential contamination as well as cell line characterization during process development and scale-up…

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